VE-cadherin (Cdh5 or Cadherin-5 or CD144) Antibody - Western Blot Validated

Protein Function

Bring VE-cadherin (CD144) into view to assess changes across conditions. VE-cadherin is a junctional protein that coordinates adhesion with cytoskeletal anchoring. Look for shifts in distribution that track with phenotype changes.

In some datasets and protocols, it is referenced by the gene symbol CD144.

Experimental Applications

In cultured models, junctional readouts can change rapidly after calcium modulation or cytoskeletal perturbation. Pair measurements with orthogonal readouts when interpretation is sensitive to context.

Many workflows benefit from tracking both signal intensity and compartment distribution, especially when the target participates in multi-protein assemblies. Endothelial model systems may track this target alongside barrier behavior and junction reorganization.

Common experimental questions that benefit from target tracking include:

• co-localization patterns with cytoskeletal elements
• changes in complex assembly with scaffold or adaptor proteins
• comparative profiling across cell states or perturbations
• subcellular redistribution at contact sites versus cytoplasm

Workflow Perspective

When samples are prepared in reducing conditions, some epitopes become more accessible; this version is designed with that context in mind. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series.

If your study spans multiple model systems, it can help to define a shared reference condition for VE-cadherin so cross-sample contrasts stay meaningful.

When interpreting VE-cadherin, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.

Choose the denaturing-format option when you want VE-cadherin (CD144) results aligned to your sample format.