VE-cadherin (Cdh5 or Cadherin-5 or CD144) (rat) Antibody

Protein Function

Use this antibody to evaluate VE-cadherin in your model system. VE-cadherin is a junctional protein that coordinates adhesion with cytoskeletal anchoring. Focus on trends across conditions rather than single-point snapshots.

In some datasets and protocols, it is referenced by the gene symbol CD144.

Experimental Applications

In cultured models, junctional readouts can change rapidly after calcium modulation or cytoskeletal perturbation. Pair measurements with orthogonal readouts when interpretation is sensitive to context.

Many workflows benefit from tracking both signal intensity and compartment distribution, especially when the target participates in multi-protein assemblies. Blood-brain barrier models often monitor junction regulation alongside permeability-focused measurements.

Common experimental questions that benefit from target tracking include:

• co-localization patterns with cytoskeletal elements
• comparative profiling across cell states or perturbations
• changes in complex assembly with scaffold or adaptor proteins
• subcellular redistribution at contact sites versus cytoplasm

Workflow Perspective

This regular format is a practical starting point when you want flexibility across sample types and iterative optimization. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series.

When interpreting VE-cadherin, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.

To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so VE-cadherin patterns can be compared across experiments without guesswork.

With a clear focus on VE-cadherin, this regular format option fits streamlined studies and fast iteration.