VE-cadherin (Cdh5 or Cadherin-5 or CD144) (rat) Antibody - FFPE Validated

Scientific Overview

Build reproducible readouts by using this antibody to evaluate VE-cadherin across matched sample sets. VE-cadherin is a membrane-associated component that helps organize cell-cell contacts and polarity. Use matched handling so differences reflect biology rather than processing.

In some datasets and protocols, it is referenced by the gene symbol CD144.

Research Relevance

In cultured models, junctional readouts can change rapidly after calcium modulation or cytoskeletal perturbation. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.

When cell-state transitions occur, this target may change alongside partners in the same compartment or pathway neighborhood. Blood-brain barrier models often monitor junction regulation alongside permeability-focused measurements.

When building a narrative around the target, it helps to track:

• changes in complex assembly with scaffold or adaptor proteins
• comparative profiling across cell states or perturbations
• subcellular redistribution at contact sites versus cytoplasm
• co-localization patterns with cytoskeletal elements

Application-Specific Context

This fixed-section oriented version is intended for paraffin-embedded tissue, where crosslinking and retrieval steps can shape epitope accessibility. Think about how preparation affects epitope accessibility, and interpret patterns alongside morphology and compartment markers as needed. A short pilot series can help set exposure ranges and reduce reruns.

For challenging targets, pairing VE-cadherin measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.

For multi-run studies, the fixed-section option helps keep VE-cadherin trends comparable from experiment to experiment.