WNK1 Antibody
BiCell Scientific
SKU:50001-50UL
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Protein Function
This antibody helps you assess WNK1 while keeping condition-to-condition contrasts straightforward. WNK1 is a pathway component whose activation state and localization can change after stimulation. For comparison-heavy studies, consistent sample handling and normalization help reduce run-to-run drift.
Following WNK1 across perturbations can help separate expression effects from compartment shifts.
Experimental Applications
Parallel measurement of upstream inputs and downstream readouts can sharpen interpretation of pathway behavior. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.
Many workflows benefit from tracking both signal intensity and compartment distribution, especially when the target participates in multi-protein assemblies.
Common experimental questions that benefit from target tracking include:
- localization shifts between cytoplasm, membrane, and nucleus
- interaction patterns within multi-protein complexes
- condition-to-condition comparisons with matched controls
- time-resolved changes following stimulation or inhibition
Workflow Perspective
When assay conditions are still being tuned, a regular-format antibody supports stepwise adjustment of buffers and incubation steps. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series. Document normalization choices so collaborators can reproduce the same interpretation.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so WNK1 patterns can be compared across experiments without guesswork.
For multi-run studies, the regular format option helps keep WNK1 trends comparable from experiment to experiment.
- Targets:
- WNK1
- Research Area:
- Cell Signaling
- Application:
- IF • IHC • WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rabbit
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (17-aa) derived from the C-terminal region of human WNK1 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q9H4A3
- Homology:
- Synthetic peptide sequence is identical to mouse or rat sequence
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C